H5N1/H9N2-mix study

Message Topic Search Topic Options Post Reply Create New Topic Printable Version Translate Topic

   http://www.sciencedirect.com/science/article/pii/S0378113516301791
A/Goose/Jiangsu/k0403/2010 [GS/10(H5N1)] virus was used as a backbone to generate a series of reassortants, each contained a single internal gene derived from the predominant S genotype of the A/Chicken/Jiangsu/WJ57/2012 [WJ/57(H9N2)]. We next assessed the biological characteristics of these assortments, including pathogenicity, replication efficiency and polymerase activity. We found that the parental WJ/57(H9N2) and GS/10(H5N1) viruses displayed high genetic compatibility. Notably, the H5N1 reassortants containing the PA or NP gene from WJ/57(H9N2) virus significantly increased virulence and replication ability in mice, as well as markedly enhanced polymerase activity. Our results indicate that the endemicity of H9N2 and H5N1 in domestic poultry greatly increases the possibility of generating new viruses by reassortment that may pose a great threat to poultry industry and public health.

Author	Message Topic Search Topic Options Post Reply Create New Topic Printable Version Translate Topic
Dutch Josh Members Profile Send Private Message Find Members Posts Add to Buddy List Adviser Group Joined: May 01 2013 Location: Arnhem-Netherla Status: Offline Points: 95387	Post Options Post Reply Quote Dutch Josh Report Post Thanks(0) Quote Reply Topic: H5N1/H9N2-mix study Posted: August 20 2016 at 10:23pm
	http://www.sciencedirect.com/science/article/pii/S0378113516301791 A/Goose/Jiangsu/k0403/2010 [GS/10(H5N1)] virus was used as a backbone to generate a series of reassortants, each contained a single internal gene derived from the predominant S genotype of the A/Chicken/Jiangsu/WJ57/2012 [WJ/57(H9N2)]. We next assessed the biological characteristics of these assortments, including pathogenicity, replication efficiency and polymerase activity. We found that the parental WJ/57(H9N2) and GS/10(H5N1) viruses displayed high genetic compatibility. Notably, the H5N1 reassortants containing the PA or NP gene from WJ/57(H9N2) virus significantly increased virulence and replication ability in mice, as well as markedly enhanced polymerase activity. Our results indicate that the endemicity of H9N2 and H5N1 in domestic poultry greatly increases the possibility of generating new viruses by reassortment that may pose a great threat to poultry industry and public health.
	We cannot solve our problems with the same thinking we used when we created them. ~Albert Einstein

Dutch Josh Members Profile Send Private Message Find Members Posts Add to Buddy List Adviser Group Joined: May 01 2013 Location: Arnhem-Netherla Status: Offline Points: 95387	Post Options Post Reply Quote Dutch Josh Report Post Thanks(0) Quote Reply Posted: August 20 2016 at 10:31pm
	http://www.wallstreetdaily.com/2016/04/21/china-europe-silk-road-railway/ The new railroad linking Europe with China (via Russia and Kazachstan) makes travel of goods (and virusses, bacteria etc) a two week train ride in stead of a month on a ship. (DJ) Incubationtime differ a lot from diseases. But the shorter the traveltime the faster an illness can be spread before the danger is realized. Of course travel by air is already a major risk. A containership can carry more containers than a train can. But will we get transports from SE Asia of life animals, being slaughtered and packed in Kazachstan to get consumed in Europe ?
	We cannot solve our problems with the same thinking we used when we created them. ~Albert Einstein