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Vaccinia Proposed Cancer Treatment Study Abstract |
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Technophobe
Assistant Admin Joined: January 16 2014 Location: Scotland Status: Offline Points: 88450 |
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Posted: March 03 2016 at 2:35pm |
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Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrickTM design, serum-free virus production and microcarrier-based cultivation of CV-1 cells.Shuchang Liu, Ludmila Ruban, Yaohe Wang, Yuhong Zhou, Darren N. Nicholas Nesbeth doi: http://dx.doi.org/10.1101/042275 This article is a preprint and has not been peer-reviewed [what does this mean?]. AbstractVaccinia virus (VACV) is an established tool for vaccination and is beginning to prove effective as an oncolytic agent. Industrial production of VACV stands to benefit in future from advances made by synthetic biology in genome engineering and standardisation. As a step toward realising these benefits we analysed the Lister Vaccinia virus genome with respect to refactoring options and propose a VACV genome engineering BioBrickTM. We then used the CV-1 cell line to produce a conventional recombinant Lister strain VACV, VACVL-15 RFP in a serum-free process. CV-1 cells grown in 5% foetal bovine serum (FBS) Dulbecco's Modified Eagle Medium (DMEM) were adapted to growth in OptiPRO and VP-SFM brands of serum-free media. Specific growth rates of 0.047 h-1 and 0.044 h-1 were observed for cells adapted to OptiPRO and VP-SFM respectively, compared to 0.035 h-1 in 5% FBS DMEM. Cells adapted to OptiPRO and to 5% FBS DMEM achieved recovery ratios of over 96%, an indication of their robustness to cryopreservation. Cells adapted to VP-SFM showed a recovery ratio of 82%. VACV production in static culture, measured as plaque forming units (PFU) per propagator cell, was 75 PFU/cell for cells in 5% FBS DMEM. VP-SFM and OptiPRO adaptation increased VACV production to 150 PFU/cell and 350 PFU/cell respectively. Boosted PFU/cell from OptiPRO-adapted cells persisted when 5% FBS DMEM or OptiPRO medium was present during the infection step and when titre was measured using cells adapted to 5% FBS DMEM or OptiPRO medium. Finally, OptiPRO-adapted CV-1 cells were successfully cultivated using Cytodex-1 microcarriers to inform future scale up studies. A quick explanation for the hopelessly lost - from one only partially confused: A proposed treatment for cancer involving making a virus target and kill the cancerous cells. This is proving doubly effective as it both targets the nasties directly and encourages the host's immune system to fight them too. This article details new production methods which double/quadrouple the viral production in improved growing mediums and shows that they survive cryogenic storage. Vaccinia = (a horse virus related to cowpox which was the first smallpox vaccine)
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Technophobe
Assistant Admin Joined: January 16 2014 Location: Scotland Status: Offline Points: 88450 |
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Less than an hour after I posted that, The Times posted this: http://www.thetimes.co.uk/tto/science/article4705309.ece which is an article suggesting that immunotherapy could be the magic bullet we are all praying for. Sorry all, I do not have a subscription so I cannot post the full article, but the gist is lovely!
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How do you tell if a politician is lying?
His lips or pen are moving. |
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Technophobe
Assistant Admin Joined: January 16 2014 Location: Scotland Status: Offline Points: 88450 |
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Luckily someone at the Mail is posting the same story.....
A cancer cure in just one jab? British scientists say they have found the disease's 'Achilles heel' paving the way for 'revolutionary' new treatments.
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How do you tell if a politician is lying?
His lips or pen are moving. |
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