Tracking the next pandemic: Avian Flu Talk |
Evidence suggests new bird flu spread among people |
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Albert
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Posted: August 06 2013 at 4:51pm |
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Evidence suggests new bird flu spread among people
AP
7:40 p.m. EDT August 6, 2013 LONDON (AP) — Chinese scientists have found the strongest evidence yet that a new bird flu strain is sometimes able to spread from person to person, but they are emphasizing that the virus still does not transmit easily. The new bird flu strain, known as H7N9, was first reported by Chinese authorities in March. As of the end of May, there were 132 cases and 37 deaths in China and Taiwan linked to the virus. Health officials suspect patients were most likely infected by birds in live animal markets but acknowledged there were probably sporadic cases of the virus spreading among humans. Except for a single case reported last month, the infections appear to have stopped since Chinese authorities took measures to slow the virus, including shutting down live markets across the country. In the new study, Chinese researchers interviewed the family and close friends of a father and daughter both killed by H7N9 in eastern China to figure out how the virus might have spread between them. Both patients lived in the same household, were critically ill during the investigation and could not be interviewed. The father, 60, was in charge of buying food for the family and bought six live quails before falling sick. His daughter, 32, rarely left the residential district where they lived and didn't have any known contact with birds, except for two black swans raised by the property owners. The daughter took care of her father when he became ill, without wearing any protective equipment. She fell sick several days afterward and died one month later. The bird flu viruses isolated from the father and daughter were nearly genetically identical. There is no definitive test to prove when a virus has spread from human-to-human, but scientists consider matching viruses and eliminating other ways the virus might have spread to be convincing evidence. Scientists also tested 43 contacts of the two patients; none had H7N9. "In this cluster, the virus was able to transmit from person-to-person," wrote Xian Qi of the Jiangsu Province Center for Disease Control and Prevention, who was the lead author of the study. The scientists concluded the transmission was "limited and non-sustainable." The paper was published online Tuesday in the journal BMJ. "It is also notable that the transmission occurred between blood relatives," said Dr. Peter Horby, a bird flu expert at Oxford University Clinical Research Unit in Hanoi, Vietnam, in a statement. Horby, who was not involved in the latest study, noted there is some evidence that genetic factors may make some people more susceptible to bird flu. In an accompanying commentary in the BMJ, experts said similar patterns had been seen with other types of bird flu, including H5N1, another feared bird flu strain that first emerged in 1996 and has since killed millions of chickens. It has sickened more than 600 people and caused 377 deaths, mostly in Asia. "To observe some transmission of H7N9 from human-to-human...does not necessarily indicate the virus is on course" to spark a pandemic, wrote James Rudge of the London School of Hygiene and Tropical Medicine, who is based at Mahidol University in Thailand, and Richard Coker of the National University of Singapore. Still, Rudge and Coker noted several worrying features about H7N9, such as its ability go undetected in birds before sickening humans. They also warned officials to be on guard for a possible return of H7N9 in the winter; flu viruses typically spread more easily in cold weather. "The threat posed by H7N9 has by no means passed," they said. http://www.usatoday.com/story/news/health/2013/08/06/bird-flu-spread/2625877/ |
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Albert
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I think we all knew awhile ago that there was limited h2h, and quite a bit of it.
It could actually cause a pandemic - and be a slow moving one. In this case, it may not need to achieve full sustained human to human transmission to become a pandemic as it will probably slowly spread this winter. On the other side of the coin, it will also give h7 a lot of opportunities to mix with h1n1 and breed a hybrid flu. |
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cobber
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The article's a bit late to the party. (better late than never)
Found a similar article via my favorite news site DRUDGE, and they sourced this one from Reuters H7N9 bird flu in likely China spread between people, researchers findKate KellandReuters 1 hour ago LONDON - The first scientific analysis of probable human-to-human transmission of a deadly new strain of bird flu that emerged in China this year gives the strongest evidence yet that the H7N9 virus can pass between people, scientists said on Wednesday. Research published in the British Medical Journal (BMJ) analyzing a family cluster of cases of H7N9 infection in eastern China found it was very likely the virus "transmitted directly from the index patient (a 60-year-old man) to his daughter." Experts commenting on the research said while it did not necessarily mean H7N9 is any closer to becoming the next flu pandemic, "it does provide a timely reminder of the need to remain extremely vigilant." "The threat posed by H7N9 has by no means passed," James Rudge and Richard Coker of the London School of Hygiene and Tropical Medicine said in a commentary in the same journal. The scientists who led the study stressed, however, that the virus has not yet gained the ability to transmit from person to person efficiently - meaning the risk is very low that it could cause a human pandemic in its current form. The new bird flu virus, which was unknown in humans until February, has so far infected at least 133 people in China and Taiwan, killing 43 of them, according to the latest World Health Organization (WHO) data. Most cases have been in people who had visited live poultry markets or had close contact with live poultry in seven to 10 days before falling ill. The BMJ study, lead by Chang-jun Bao at the Jiangsu Province Centre for Disease Control and Prevention, analyzed a family cluster of two H7N9 patients - a father and daughter - in Eastern China in March 2013. The first "index" patient - a 60-year-old man - regularly went to a live poultry market and became ill five to six days after his last exposure to poultry. He was admitted to hospital on March 11. When his symptoms became worse, he was transferred to an intensive care unit (ICU) on March 15 but died of multi-organ failure on May 4, the study reported. The second patient, his healthy 32-year-old daughter, had no known exposure to live poultry but provided direct bedside care for her father in the hospital before he went to intensive care. She developed symptoms six days after her last contact with her father and went into hospital on March 24. She was moved to the ICU on March 28 and died of multi-organ failure on April 24. Strains of the virus isolated from samples taken from each patient were "almost genetically identical" - a strong suggestion that the virus was transmitted directly from father to daughter, the researchers said. "To our best knowledge, this is the first report of probable transmissibility of the novel virus person-to-person with detailed epidemiological, clinical and virological data," they wrote. Peter Horby of the Oxford University clinical research unit in Hanoi, Vietnam, who was not involved in this research, said the study raised the level of concern about H7N9 and reinforced the need for intensive surveillance. Copyright 2013 Thomson Reuters. Gold Star for BMJ. They are just confirming what we have discussed here for months. There have been several other tests which have come to similar conclusions. Mainstream media focus is good. Hopefully groups like the WHO and the alike can attract more funding and actually afford decent programs to mitigate these viruses. While i'm here on the topic... Big ups to Billy Gates for for his philanthropy in this area. He is the second largest contributor to the WHO. Cheers! |
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cobber
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Here's the study by BMJ
http://www.bmj.com//347/bmj.f4752 - http://www.bmj.com/content/347/bmj.f4752 AbstractIntroductionA novel avian influenza A (H7N9) virus was identified recently in Eastern China. http://www.bmj.com//347/bmj.f4752#ref-1 - 1 As of 30 May 2013, a total of 132 cases were reported, distributed sporadically in 10 provinces/municipalities with one case in Taiwan. http://www.bmj.com//347/bmj.f4752#ref-2 - 2 In most of the laboratory confirmed cases the patients developed severe pneumonia and acute respiratory distress syndrome (ARDS) and needed intensive care. http://www.bmj.com//347/bmj.f4752#ref-3 - 3 Thirty seven died of respiratory failure or other complications. Human infections with this virus had not been reported before. Infections with other H7 subtypes, such as H7N2, H7N3, and H7N7, which are usually related to outbreaks of poultry, have been reported in several countries. http://www.bmj.com//347/bmj.f4752#ref-4 - 4 These subtypes, however, often cause only mild to moderate disease, and only one fatal case has been reported (Netherlands in 2003). http://www.bmj.com//347/bmj.f4752#ref-5 - 5 According to the current available epidemiological data, most affected patients had a history of visiting live poultry markets or contact with poultry seven to 10 days before the onset of illnesses, indicating that the sources of infection were likely to be either contaminated environment or infected poultry. http://www.bmj.com//347/bmj.f4752#ref-6 - 6 http://www.bmj.com//347/bmj.f4752#ref-7 - 7 http://www.bmj.com//347/bmj.f4752#ref-8 - 8 http://www.bmj.com//347/bmj.f4752#ref-9 - 9 Until now, no clear evidence indicated that the novel virus could transmit from person to person. http://www.bmj.com//347/bmj.f4752#ref-10 - 10 Family clusters with confirmed or suspected avian H7N9 virus infection were previously reported http://www.bmj.com//347/bmj.f4752#ref-11 - 11; however, some information related to the cluster in Jiangsu Province was incomplete and even incorrect. Humans seemed to be more susceptible to the H7N9 virus than the H5N1 virus. http://www.bmj.com//347/bmj.f4752#ref-12 - 12 It is therefore vital to establish whether the novel virus can transmit from person to person because of the potential threat of a pandemic if it possesses sustainable transmissibility between humans. http://www.bmj.com//347/bmj.f4752#ref-13 - 13 http://www.bmj.com//347/bmj.f4752#ref-14 - 14 We report a family cluster of two patients with novel avian H7N9 virus infection. Video abstract MethodsEpidemiological investigation and sample collectionPublic health staff interviewed all family members and doctors and nurses who provided service for the two patients on multiple occasions to validate timelines of events and, in particular, to verify possible exposure history before the onset of illnesses, including raising or contact with animals, visiting live poultry markets and purchase of live poultry, and contact with febrile patients. We could not interview the two patients because they were critically ill at the time of investigation. In addition to the household and surrounding environments (the residential district, which was about 1 million square metres) where the two patients lived, live poultry markets and convenience stores the index patient visited were all inspected to assess potential exposures to poultry and the environment. We reviewed medical records to determine the time of onset and progression of the illness. All household members and healthcare workers who had contact with the two patients within 1 metre without effective personal protection at any time from the day before illnesses onset to when the patients were isolated in hospitals were treated as close contacts and placed under medical observation for seven days. Paired serum samples (separated by at least three weeks) were collected to ascertain potential person to person transmission as well as asymptomatic and subclinical infections. Clinical samples for laboratory testing included samples from the two patients on 27 and 31 March, 13 environmental samples including two smears from chicken cages, three smears from pigeon cages, two samples of chicken faeces, one sample of pigeon faeces, one sample of duck faeces, two cloacal swabs from chicken, and two sewer water samples from two live poultry markets on April 2, and two cloacal swabs from swans on 5 April from the residential area where the two patients lived. Laboratory investigationSpecimens in viral transport medium were tested by real time reverse transcriptase-polymerase chain reaction (rRT-PCR) analysis and by inoculation into Madin-Darby canine kidney cell (MDCK) culture for viral isolation, including one to three blind passages, as previously described. http://www.bmj.com//347/bmj.f4752#ref-1 - 1 Viral RNA was reverse transcribed into cDNA with SuperScript III First-Strand Synthesis System (Invitrogen, Life Technologies, Carlsbad, CA, USA) with random hexamers. Double strand cDNA was synthesised with Sequenase 2.0 (USB, Affymetrix, Cleveland, OH, USA). Sequencing libraries were prepared with the Nextera XT DNA sample preparation Kit (Illumina, San Diego, CA, USA). Samples from the second patient and the environment were pooled together and then run on Illumina MiSeq platform to generate 150 bp paired end reads. Assembly of viral reads was carried out with influenza A isolate A/Anhui/1/2013(H7N9) as the reference genome (GISAID, accessions EPI439503-EPI439510). In addition, full genomes from the index case were amplified with previously described methods http://www.bmj.com//347/bmj.f4752#ref-15 - 15and sequenced on ABI 3130 automatic DNA analyser (Life Technologies) with ABI BigDye Terminator v3.1 cycle sequencing kit (Life Technologies). Full genome sequences of the viruses were deposited in GenBank (accession number: http://www.bmj.com/lookup/external-ref?_=GEN&access_num=KF034916&atom=%2Fbmj%2F347%2Fbmj.f4752.atom - KF034916- http://www.bmj.com/lookup/external-ref?_=GEN&access_num=KF034923&atom=%2Fbmj%2F347%2Fbmj.f4752.atom - KF034923 for the father (index case), http://www.bmj.com/lookup/external-ref?_=GEN&access_num=KF034908&atom=%2Fbmj%2F347%2Fbmj.f4752.atom - KF034908- http://www.bmj.com/lookup/external-ref?_=GEN&access_num=KF034915&atom=%2Fbmj%2F347%2Fbmj.f4752.atom - KF034915 for the daughter, and http://www.bmj.com/lookup/external-ref?_=GEN&access_num=KF150605&atom=%2Fbmj%2F347%2Fbmj.f4752.atom - KF150605- http://www.bmj.com/lookup/external-ref?_=GEN&access_num=KF150612&atom=%2Fbmj%2F347%2Fbmj.f4752.atom - KF150612 for the environment). Sequences were compiled with the Lasergene sequence analysis software package (DNAStar, Madison, WI, USA). Nucleotide BLASTn analysis was used to identify related reference strains, and reference sequences were obtained from GeneBank and GISAID. Pairwise sequence alignments were also performed with the MegAlign program (DNASTAR) to determine similarities between nucleotide and amino acid sequence. Phylogenetic analysis of the aligned sequences for eight genomic segments was performed by the maximum likelihood methods with MEGA4.1 software. http://www.bmj.com//347/bmj.f4752#ref-16 - 16 The reliability of the unrooted neighbour-joining tree was assessed by bootstrap analysis with 1000 replications; only bootstrap values ≥70 % are shown. Horizontal distances are proportional to genetic distance. Alignments of each influenza virus sequence were created with the program ClustalX 1.83. Serum samples were tested in a modified in house haemagglutination inhibition assay with turkey erythrocytes. http://www.bmj.com//347/bmj.f4752#ref-17 - 17 Antigens for the assays were produced from the A/Nanjing/1/2013(H7N9) strain isolated from the confirmed case in Jiangsu. http://www.bmj.com//347/bmj.f4752#ref-8 - 8 All testing was performed at the BSL-2 or BSL-3 laboratories of Jiangsu Province Center for Disease Control and Prevention, Nanjing, China. ResultsPatientsThe index patient was a retired man aged 60 with a history of hypertension for more than 10 years. He developed a fever, cough, and shortness of breath on 8 March 2013 and was admitted to a Chinese hospital (hospital A) on March 11 with a left upper lobe inflammation. Initial blood routine testing identified no abnormality, except for increased hypersensitive C reactive protein (28.5 mg/L). He was treated with azithromycin and piperacillin-sulbactam. Because of progressive respiratory distress, persistent hyperpyrexia, and hypoxemia, he was transferred to the hospital’s intensive care unit in the afternoon of 15 March with a diagnosis of viral pneumonitis and type I acute respiratory distress syndrome. He was again transferred to another tertiary hospital’s (hospital B) intensive care unit because of deterioration on 18 March and began to treatment with oseltamivir the next day. He did not develop diarrhoea during the course of the disease. He died of disseminated intravascular coagulation and multi-organ failure on 4 May. The index patient’s daughter, an unemployed woman aged 32, was otherwise healthy without any underlying illnesses. She provided bedside care for her father until he was admitted to the second hospital’s intensive care unit. She developed fever with body temperature 39.6°C and cough on 21 March. On 24 March, she was admitted to the pneumology department of the same hospital (hospital B) with pneumonia in the left upper lobe. Initial testing showed leucocytopenia (2.0×109/L), lymphopenia (0.7×109/L), and slight hypoxia. She was treated with antibiotics (azithromycin and piperacillin-sulbactam). Oseltamivir (75 mg twice a day) was administered on 24 March. She was transferred to intensive care on 28 March because of persistent hyperpyrexia, respiratory failure, and acute respiratory distress syndrome. Although treated with mechanical ventilation, broad spectrum antibiotics, oseltamivir, immunological therapy, and fluid resuscitation, she died of multi-organ failure and cardiac arrest on 24 April. Table A in appendix 1 summarises the clinical characteristics of the two patients. Epidemiological investigationsThe index patient lived in an elite residential district with his wife, daughter, son in law, and granddaughter. An ornamental pool was located in the centre of the residential district. With the exception of two black swans that were raised by property management personnel for about two years, there were no other fowl or poultry in the residential district. There were two convenience stores on the edge of the residential district located less than 500 metres from the home where only frozen but not live poultry were sold. About 2 km away from the residential district, there were two free live markets where several kinds of live poultry were sold. Interviews with the other family members and doctors and nurses who provided services for the two patients permitted reconstruction of a timeline for the index patient and his daughter (figure). http://www.bmj.com//347/bmj.f4752#F1 - ⇓ The index patient was in charge of purchasing foodstuff for the whole family every day. As a result, he often visited convenience stores and occasionally live markets. In particular, he purchased six quails in one of the live markets and cooked for the family one day between 1 March and 4 March. The exact date was unclear. The second patient was unemployed, and her daily routine was to take care of her daughter aged 22 months. She rarely walked beyond her residential district. From the time the index patient became ill until he was admitted to the second hospital’s intensive care unit, a period of about 10 days, the daughter provided much of the care for her father between 8 and 10 March at home (conventional care) and in the afternoons between 11 and 13 March (visiting), all day on 14 March (bedside care), and in the morning of 15 March (bedside care) in the hospital, including bedside care for six to eight hours a day. From 14 March, her father had abundant expectoration. More importantly, aside from conventional care, she was in charge of cleaning her father’s secretions and maintaining his oral hygiene during care without any personal protective equipment. The son in law provided bedside care for the index patient during other time periods. The father was transferred to intensive care in the afternoon of 15 March. After then, she visited her father once a day for about half an hour in the afternoons from 16 to 18 March wearing a mask and had no close contact with her father after 18 March because he was transferred to an isolated single unit, where she visited her father only through a glass window. http://www.bmj.com/highwire/stream/656694/field_highwire_fragment__l/0/F1.medium.gif - http://www.bmj.com/highwire/stream/656694/field_highwire_fragment__m/0/F1.medium.gif View larger version:
Timeline of family cluster of two patients with novel avian influenza A (H7N9) virus infection in Eastern China, 2013. Strain isolation used Madin-Darby canine kidney cell culture We drew up a full list of potential contacts, including everyone who had contact with the patients from the onset of their illnesses till effective isolation on 2 April, and local public health officials then interviewed individuals. We identified 43 close contacts, including 39 healthcare workers, three household members, and one relative. Among the 39 healthcare workers, 15 came from hospital A and were close contacts of the index case; 24 came from hospital B; 14 were close contacts of the daughter, and 10 were close contacts of both patients (see table B in appendix 1). The main types of contact for patients were physical examination and various medical services. Contact with patients took place at least once a day. The mean number of days of contact with patients was four (range 1-8 days). Other than the husband of the second case, who had a slight fever (37.5°C) on 31 March, no other close contacts developed febrile respiratory symptoms. He was treated with oseltamivir, and his throat swab sample tested negative for influenza A viruses by rRT-PCR with universal primers. All other 42 close contacts did not receive prophylactic antiviral drug after exposure. No haemagglutination inhibition antibodies against A (H7N9) virus were detected from paired serum samples of all close contacts. Higher titres of haemagglutination inhibition antibodies, however, were observed from both patients (figure). http://www.bmj.com//347/bmj.f4752#F1 - ⇑ Laboratory investigationsrRT-PCR analysis of an endotracheal aspirate specimen collected on 31 March from the index patient indicated that it contained weak novel avian H7N9 nucleic acid, although no positive results were obtained from a throat swab sample collected on 27 March. Both throat swab and endotracheal aspirate samples collected on 27 March and 31 March from the daughter were also positive for avian H7N9 viral genes. Infection with other respiratory pathogens, such as SARS-CoV, novel CoV, seasonal influenza viruses (classic H1, novel H1pdm09, H3, or B), and avian influenza H5N1 and H9N2, was not detected. Three strains—two from samples collected on 31 March from the index and subsequent patient (figure http://www.bmj.com//347/bmj.f4752#F1 - ⇑) and one from a smear sample forma poultry cage collected on 2 April from one of the two live poultry markets that the index patient often visited—were isolated successfully. Sequences of all eight genes of the two strains isolated from patients were nearly identical (similarity ranged from 99.6% to 99.9%) but slightly different from the strain isolated from environment (table) http://www.bmj.com//347/bmj.f4752#T1 - ⇓. In addition, sequencing of the two strains from patients showed that all the viral genes were avian and were closely related to other human A (H7N9) viral sequences previously identified in Shanghai, Zhejiang, and Anhui Province but genetically different from strains isolated from the environment and birds. Further phylogenetic analysis showed all eight genes of the two strains from patients belonged to the same clade (see appendix 2). View this table:
Nucleotide sequences identities among eight genes of three strains* isolated from two patients and environment related to family cluster of novel avian influenza A (H7N9) virus infection in Eastern China, 2013 Analysis of the key functional amino acid sites in different viral proteins associated with interspecies transmission or drug resistance of the three isolates showed that both strains isolated from patients were the same as the strains of Shanghai/2, Anhui/1, and Nanjing/1 but somewhat different from strain of Shanghai/1, which had R294K of NA and isoleucine (I) but not valine (V) at 368 site of PB1. In addition, the two strains isolated from the patients had E627K; however, the strain isolated from environment still possessed E at the same site (see table C in appendix 1). DiscussionWe believe that the most likely explanation for this family cluster of the two patients with novel avian influenza H7N9 virus infection is that the virus transmitted directly from the index patient to his daughter. Firstly, the diagnosis of the two patients was confirmed virologically, and the clinical features―fever, pneumonia with lymphopenia, and rapid progression to acute respiratory distress syndrome—all correspond to the cardinal features of reported cases in humans in China. http://www.bmj.com//347/bmj.f4752#ref-17 - 17 http://www.bmj.com//347/bmj.f4752#ref-18 - 18Secondly, it was fortuitous for the investigation that the daughter did not visit the markets to buy foodstuffs and cook for the family and had no exposure to animals or history of visiting live poultry markets. She did, however, have prolonged, direct, and unprotected exposure to her father. Thirdly, two strains were isolated successfully from the two patients. Further sequence analysis showed that both possessed high degrees of similarity between nucleotide (99.6%-99.9%) and amino acid (99.0%-100%) sequences. Finally, before this study, A (H7N7) among H7 subtype influenza viruses and H5N1 virus was known to have the ability to transmit from person to person. http://www.bmj.com//347/bmj.f4752#ref-19 - 19 http://www.bmj.com//347/bmj.f4752#ref-20 - 20 http://www.bmj.com//347/bmj.f4752#ref-21 - 21 Animal (ferrets and pigs) experiments indicated that the H7N9 virus possessed the capability to bind to both avian and human receptor and to transmit itself by droplet under certain conditions. http://www.bmj.com//347/bmj.f4752#ref-22 - 22 http://www.bmj.com//347/bmj.f4752#ref-23 - 23 Our findings, however, indicated that the virus has not gained the ability for efficient sustained transmission from person to person. From 14 March, the daughter came into direct contact with the oral secretions of the index patient without any personal protective equipment, contacting the patient at a much higher rate than other individuals in contact with the patient. Similar to other available human strains, the characteristics of the two strains showed no adaptation change in the receptor binding site from the avian 2,3-linked pattern toward the human 2,6-linked pattern of sialic acid receptor. http://www.bmj.com//347/bmj.f4752#ref-1 - 1 http://www.bmj.com//347/bmj.f4752#ref-7 - 7 Phylogenic tree analysis of all eight genomic segments indicated that the two isolates were of avian origin and that there was no reassortment with human or swine influenza viruses. http://www.bmj.com//347/bmj.f4752#ref-24 - 24 http://www.bmj.com//347/bmj.f4752#ref-25 - 25 http://www.bmj.com//347/bmj.f4752#ref-26 - 26 Furthermore, no asymptomatic or subclinical infections were identified among 43 close contacts by haemagglutination inhibition testing. Recent studies also indicated that avian H7N9 tends to bind lower pulmonary epithelial cells rather than those of the upper respiratory tract, which makes it difficult to transmit between humans. http://www.bmj.com//347/bmj.f4752#ref-7 - 7 The two patients were blood related. The index patient’s son in law also provided bedside care for the index patient in the mornings and nights between 11 and 13 March without any personal protective equipment. Both biological and serological evidence showed that he was not infected with the H7N9 virus. These findings suggest that potential genetic susceptibility might be one of determinants and that avian influenza viruses, like H5N1, are more easily transmitted between individuals with genetic connection. http://www.bmj.com//347/bmj.f4752#ref-27 - 27 http://www.bmj.com//347/bmj.f4752#ref-28 - 28 The possible source of infection for the index case was likely to be from the live poultry market that the patient used to visit or the six quails that he bought but were slaughtered by the seller in the market one week before illness onset. One strain of avian H7N9 was isolated from environmental samples from the live poultry markets. Visiting a wet poultry market has been identified as a risk factor for human infections. http://www.bmj.com//347/bmj.f4752#ref-8 - 8 http://www.bmj.com//347/bmj.f4752#ref-9 - 9 Important strengths and differences in relation to other studiesWith respect to the possible infection period in the second patient, we cannot ascertain when person to person transmission occurred. The most likely period was from 11 to 15 March during the index patient’s admission to hospital before transfer to intensive care, especially the periods between the afternoon of 14 March and the morning of 15 March, when the index patient began to expectorate abundantly. The daughter had direct and close contact with the index case without any protection during this period. During 8-10 March, the daughter provided only conventional care for the index patient, such as taking his temperature. Transmission is unlikely to have occurred during this period as the patient had not yet to start expectoration. Thus, the most likely incubation period was six to seven days (range 6-13 days) based on the daughter’s unprotected exposure to her father. The putative incubation period was a relatively longer than that reported by Cowling and colleagues, who estimated the average incubation period to be around three days based on Weibull model as well as live poultry to human transmission. http://www.bmj.com//347/bmj.f4752#ref-29 - 29 We estimated the incubation period based on person to person transmission and one case in cluster. Implications of the studyPossible transmission routes include contact while cleaning up infected oral secretions and subsequent inoculation of mucous membranes or the respiratory tract. Some researchers suggested that the daughter might have acquired her infection during the process of washing her father’s diarrhoea-soiled underwear. http://www.bmj.com//347/bmj.f4752#ref-11 - 11We thought this to be unlikely. Firstly, clinical data showed that the index patient did not develop diarrhoea during course of disease. Secondly, the daughter wore gloves while washing. Thirdly, no nucleic acids specific for avian H7N9 were detected from the faecal samples from the father. We noted that 39 healthcare workers were identified as close contacts in this cluster, which was a little unusual. Both the two hospitals where the two patients were admitted were general hospitals rather than hospitals specialising in infectious disease. The awareness of personal protection of the healthcare workers was relatively weak. They used common surgical masks instead of N95 masks while providing medical services for the two patients. Another important factor was the relatively long time between the patients’ admission to a clear diagnosis with H7N9 virus infection. After the cluster was identified, infection control measures were initiated to prevent potential nosocomial transmission. Patients were isolated. Healthcare workers were required take standard respiratory and contact protection. Weaknesses of the studyThere are several limitations to our study. Firstly, we could not interview with the two patients as they were both critically ill. Therefore, the possibility that the daughter acquired her infection from the environment or other sources could not be completely ruled out, although we believe that it is less likely. Environmental investigation discovered that in addition to two swans raised by an employee of the property management, there were no other birds or poultry in her living environment. No positive results were detected from the cloacal swab and faecal samples from the two swans. Secondly, the sensitivity of haemagglutination inhibition assay was not satisfied. Thus, subclinical or asymptomatic infections could not be excluded among close contacts. A more sensitive method such as micro-neutralisation assay should be considered in the future. Finally, whole sequence alignment showed that the two isolates from patients were not identical. It is reasonable to expect slight differences between the two strains. Variations of the viral genomes occur constantly because of the high error rate of the viral polymerase. We sequenced viral genomes from the strain from the father that underwent three successive passages using MDCK cell lines. Evolution of the strain might have occurred in vivo because of the long time interval as well as the use of antiviral drugs. A previous study showed that influenza viruses often differ from those present in clinical specimens after isolation in MDCK cell culture as adaptive changes occur during virus transmission from the human host to cells of heterologous origin. http://www.bmj.com//347/bmj.f4752#ref-30 - 30 Unanswered questions and future researchTo the best of our knowledge, this is the first report of probable transmissibility of this novel virus from person to person with detailed epidemiological, clinical, and virological data. The importance of an isolated case of such transmission means there is potential for greater human to human transmission. Thus, timely detection as well as rapid investigation and risk assessments of clusters is critically important as the increase in clusters might indicate potential transmissibility of a novel virus. What is already known on this topic
What this study adds
NotesFootnotes
This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 3.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/3.0/ - http://creativecommons.org/licenses/by-nc/3.0/. References
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Suzi
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H7N9 infects poultry without symptoms while MERS-CoV infects doctors and nurses like crazy. Both really scary. Both CFR's are bad news.
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TheBrick
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If the Chinese are starting to come clean on this, something really big must be going on.
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cobber
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I don't see why this means something big is going on or something they aren't telling the world. Can you explain why you think this? |
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Albert
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The Chinese probably realize that there will be a surge in cases once the temps drop. Again, there were 1,500 - 27,000 suspected human cases, which means *a lot* of potential limited h2h.
China will give just enough info to avert a cover-up, and to save their poultry industry. China is going to have a very rough winter ahead, and let's hope that the world doesn't. Whether or not h7 achieves sustained transmission, i'm still guessing that it will spread well beyond China in this next round. |
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LOPPER
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It's not like the family clusters that got H7N9 didn't already give away the fact that it had limited H2H capability.
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Albert
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True, Lopper. We already knew it. The problem is, knowing the Chinese, there were probably a lot more. I'm guessing 30% - 50% of the cases they reported, or possibly more. If we remember last March, the majority of those infected had no contact with poultry, and I believe that there were only one or two incidents of infected poultry altogether at the wet markets.
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Wicker
Valued Member Joined: February 16 2006 Location: Mississippi Status: Offline Points: 242 |
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I just caught segment on CNN about this. It was full of info everyone here has been reporting and discussing for months. Nothing new was added. Nothing has been reported in the main stream media for a few months. Why now? I find it highly suspect. Just my 2 cents
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Wicker
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sally edelstein
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As new fears begin to spread about the new deadly bird flu and its transmission,
memories of the Asiatic flu scare of my childhood flood back..My
grandmother who came to stay with us once my mother came down with the
flu, was a veteran of the first and worst flu epidemic of 1918 and old
fears and suspicion born of that war had scarred my grandmother for life and her fears transmitted to me. At
a time when the public was petrified of the flu, everyday items came
under scrutiny and attack as potential cause of transmission. To read
more http://envisioningtheamericandream.com/2013/01/17/memories-of-the-mid-century-flu-outbreak-pt-ii/ - http://envisioningtheamericandream.com/2013/01/17/memories-of-the-mid-century-flu-outbreak-pt-ii/ |
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