Tracking the next pandemic: Avian Flu Talk |
WHO: "Actively ignoring recombination" |
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Posted: March 13 2006 at 5:51pm |
PB2 Gene of H1N1 Canadian Swine
Recombinomics Commentary March 13, 2006 Swine H1N1 and H1N2 sequences were recently made available at GenBank. These sequences were from a publication entitled “Identification of Human H1N2 and Human-Swine Reassortant H1N2 and H1N1 Influenza A Viruses among Pigs in Ontario, Canada (2003 to 2005)”. The paper focuses on ressortment between swine and human genes resulting in one swine isolate that has 8 human genes and several that have 1 human gene (PB1) and 7 swine genes. Reassortment occurs when two viruses infect the same host and new constellations of reshuffled genes emerge. However, when two virus infect the same host, recombination can also occur. Analysis of the sequences at GenBank identifies a series of recombination events in the PB2 gene. One of the 2004 isolates, A/swine/ Ontario/11112/04(H1N1), has an exact match with positions 728 through 1573 of the 1998 isolate, . A/Swine/North_Carolina/35922/98(H3N2). In contrast the two sequences differ at 31 positions within the first 711 positions. This distribution indicates the Ontario/11112 PB2 gene is a recombinant that has acquire a significant portion of the PB2 gene from an sequence similar to the North Carolina 1998 sequence. A more complete analysis of recombination is available for another Canadian swine isolate, A/swine/Ontario/53518/03)H1N1. The first 541 PB2 positions are an exact match with A/swine/Alberta/56626/03. Comparison of the remaining 1742 positions shows 295 differences. Thus the majority of the 3’ position of Ontario/53518 significantly diverges from Alberta/56626. However, the portion of the gene that diverges from Alberta/56626 is an exact match with A/Swine/Korea/CY02/02(H1N2). Thus, Ontario/53518 PB2 is a recombinant gene that is an exact match with Alberta/56626 for the first 541 positions, and the exact match with Korea/CY02 for the remainder of the gene. In addition the divergent portion of Alberta/56626 contains an exact match between positions 981 and 1300 with A/Swine/Tennessee/24/77 (H1N1). Thus, although the H1N1?s were isolated 26 years, there are no differences in the 320 BP stretch in the middle of the gene. The above examples provide clear evidence for recombination in the PB2 gene of recent H1N1 isolates from swine in Canada. Exact matches with portions of the gene can be found in isolates collected 6 to 26 years earlier. These data indicate the genes are not changing via random mutation, but instead change via recombination. Although the above examples of recombination are striking, there is no mention of recombination in the peer reviewed publication describing these sequences. Similarly, pronouncements by WHO and consultants indicate that H5N1 is evolving via random mutation. They fail to comment or acknowledge recombination. This limited analysis is of even greater concern because the sequences are sequestered in a private database, precluding evaluation of the new identified sequences. Moreover, WHO is using these unpublished and unavailable sequences to select new candidate pandemic vaccine targets. WHO is actively ignoring recombination. This limited analysis coupled with the sequestering of the most recent H5N1 is cause for concern. These sequences should be released immediately and analyzed for evidence of recombination, which can cover large portions of individual genes, as described above for PB2, but can also involve recombination involving sequence differences that involve a single nucleotide change. http://www.recombinomics.com/News/03130602/ H1N1_Recombination_Swine_PB2.html Last night I sent Dr. Niman abstracts that were posted in this forum regarding the Ontario swine. Good work folks! |
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Thomas Angel
V.I.P. Member Joined: February 16 2006 Location: United States Status: Offline Points: 622 |
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Those people at the WHO are clinically insane and seem hell bent for leather on keeping everyone in the dark.
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I LIKE SCARY RIDES
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virusil
V.I.P. Member Joined: February 26 2006 Location: United States Status: Offline Points: 450 |
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depopulation is the right modus operandi
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ignorance.
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Maybe that's what it's all about.. Maybe they want to lower the surplus population. |
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You two (virusil and ImAppalled) have a very pessimistic view on humanity. I
understand, but cannot agree that WHO 1) has the power to force depopulation or 2) the motivation to depopulate. Makes no sense. The good earth has its own mechanism to do this. |
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Scotty
Adviser Group Joined: March 06 2006 Location: United Kingdom Status: Offline Points: 846 |
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I'm appalled ImAppalled !
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Corn
Valued Member Joined: December 13 2005 Location: United States Status: Offline Points: 1219 |
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I prefer to actively ignore the WHO. being in promotions and productions, I see the whole who thing in a show biz fashion. Its all coriographed and scripted. not really real or impromptu. They are more worried about their image and the risk of making mistakes. Politicis at the highest level. They have an Asian in charge there. Wong wosomething whatever...no wonder they are so nice to China for withholding and lying. coencidence? Just ignore the WHO they mean nothing. If you treat them as some authoriative parent figure you will surely be left delusioned by daddys or mommies behavior. We the people are just an ugly step child to them. If they are a symbol of a world democracy funded by public contributions I am ashamed. They must recruit all their leadership from prostitutes. Knock knock WHOS there? |
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Speculation is the only tool we have with a threat that can circle the globe in 30 days. Test results&news is slow.Factor in human conditions,politics, money&bingo!The truth!Facts come after the fact.
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Corn
and you are usually so caring, polite and sophisticated...oh no that's someone else here. HD |
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Corn
Valued Member Joined: December 13 2005 Location: United States Status: Offline Points: 1219 |
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had just woke up and not had my coffee yet. gurrr!
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Speculation is the only tool we have with a threat that can circle the globe in 30 days. Test results&news is slow.Factor in human conditions,politics, money&bingo!The truth!Facts come after the fact.
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TERMS 1
Adviser Group Joined: March 13 2006 Location: United States Status: Offline Points: 172 |
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When I went to SWAT Medic school the one thing they pounded into our heads was there is no such thing as a fair fight and "If you ain't cheating you ain't trying". I guess WHO went to the same school of thought.
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Credibility is like virginity, once it is gone- it is gone forever.
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Falcon
Valued Member Joined: February 20 2006 Status: Offline Points: 684 |
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oh well we'll find out in the long run if this is what it is. If its true then this will be all bf needs to go from h2h
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I look at the stars and wonder what it would be like to touch them.
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gricha56
Valued Member Joined: March 07 2006 Location: United States Status: Offline Points: 47 |
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This is particularly urgent, since recombinent technology could provide us a vaccine for H5N1 prior to the emergence of the pandemic. Something we presently have very little hope for. I am hopeful. But that is my nature. I have been disappointed.
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