WHO: "Actively ignoring recombination"

PB2 Gene of H1N1 Canadian Swine 

Recombinomics Commentary
March 13, 2006

Swine H1N1 and H1N2 sequences were recently made available at
GenBank.  These sequences were from a publication entitled
“Identification of Human H1N2 and Human-Swine Reassortant H1N2 and
H1N1 Influenza A Viruses among Pigs in Ontario, Canada (2003 to
2005)”. 

The paper focuses on ressortment between swine and human genes
resulting  in one swine isolate that has 8 human genes and several that
have 1 human gene (PB1) and 7 swine genes.  Reassortment occurs when
two viruses infect the same host and new constellations of reshuffled
genes emerge.

However, when two virus infect the same host, recombination can also
occur.  Analysis of the sequences at GenBank identifies a series of
recombination events in the PB2 gene. One of the 2004 isolates, A/swine/
Ontario/11112/04(H1N1), has an exact match with positions 728 through
1573 of the 1998 isolate, . A/Swine/North_Carolina/35922/98(H3N2). In
contrast the two sequences differ at 31 positions within the first 711
positions. 

This distribution indicates the Ontario/11112 PB2 gene is a recombinant
that has acquire a significant portion of the PB2 gene from an sequence
similar to the North Carolina 1998 sequence.

A more complete analysis of recombination is available for another
Canadian swine isolate, A/swine/Ontario/53518/03)H1N1.  The first 541
PB2 positions are an exact match with A/swine/Alberta/56626/03. 
Comparison of the remaining 1742 positions shows 295 differences. 
Thus the majority of the 3’ position of Ontario/53518 significantly
diverges from Alberta/56626. 

However, the portion of the gene that diverges from Alberta/56626 is an
exact match with A/Swine/Korea/CY02/02(H1N2). 

Thus, Ontario/53518 PB2 is a recombinant gene that is an exact match
with Alberta/56626 for the first 541 positions, and the exact match
with Korea/CY02 for the remainder of the gene.  In addition the divergent
portion of Alberta/56626 contains an exact match between positions 981
and 1300 with A/Swine/Tennessee/24/77 (H1N1). Thus, although the
H1N1?s were isolated 26 years, there are no differences in the 320 BP
stretch in the middle of the gene.

The above examples provide clear evidence for recombination in the PB2
gene of recent H1N1 isolates from swine in Canada.  Exact matches with
portions of the gene can be found in isolates collected 6 to 26 years
earlier.  These data indicate the genes are not changing via random
mutation, but instead change via recombination.

Although the above examples of recombination are
striking, there is no mention of recombination in the peer reviewed
publication describing these sequences.  Similarly, pronouncements by
WHO and consultants indicate that H5N1 is evolving via random
mutation.  They fail to comment or acknowledge recombination.

This limited analysis is of even greater concern because the sequences
are sequestered in a private database, precluding evaluation of the new
identified sequences.  Moreover, WHO is using these unpublished and
unavailable sequences to select new candidate pandemic vaccine targets.

WHO is actively ignoring recombination. This limited analysis coupled
with the sequestering of the most recent H5N1 is cause for concern. 

These sequences should be released immediately and analyzed for
evidence of recombination, which can cover large portions of individual
genes, as described above for PB2, but can also involve recombination
involving sequence differences that involve a single nucleotide change.



http://www.recombinomics.com/News/03130602/
H1N1_Recombination_Swine_PB2.html


Last night I sent Dr. Niman abstracts that were posted in this forum
regarding the Ontario swine. Good work folks!

depopulation is the right modus operandi

You two (virusil and ImAppalled) have a very pessimistic view on humanity. I
understand, but cannot agree that WHO 1) has the power to force
depopulation or 2) the motivation to depopulate.

Makes no sense.

The good earth has its own mechanism to do this.

I prefer to actively ignore the WHO.

being in promotions and productions, I see the whole who thing in a show biz fashion. Its all coriographed and scripted. not really real or impromptu. They are more worried about their image and the risk of making mistakes. Politicis at the highest level.

They have an Asian in charge there. Wong wosomething whatever...no wonder they are so nice to China for withholding and lying. coencidence?

Just ignore the WHO they mean nothing.

If you treat them as some authoriative parent figure you will surely be left delusioned by daddys or mommies behavior. We the people are just an ugly step child to them.

If they are a symbol of a world democracy funded by public contributions I am ashamed.

They must recruit all their leadership from prostitutes.

Knock knock

WHOS there?

had just woke up and not had my coffee yet. gurrr!

oh well we'll find out in the long run if this is what it is.  If its true then this will be all bf needs to go from h2h